Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells
3.9K Views
•
10:46 min
•
June 14th, 2022
DOI :
June 14th, 2022
•Chapters in this video
0:05
Introduction
0:40
Thawing of Cryopreserved iPSC-Derived Airway Basal Cells (iBCs)
2:43
Dissociation of Spheroids and Expansion of iBCs in 3-D Culture
4:55
Evaluation and Purification of NGFR and iBCs
6:10
Generation of Mucociliary Air-Liquid Interface (ALI) Cultures
8:37
Cryopreservation of iBCs (Optional but Recommended)
9:15
Results: Induced Pluripotent Stem Cells for the Derivation of iBCs and ALI Cultures
10:16
Conclusion
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