This method can help answer key questions regarding the genetic regulation of the formation and maintenance of the blood-brain barrier during multiple stages of drosophila development. This method can also be adapted to examine the permeability of
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Blood-brain barrier integrity is critical for nervous system function. In Drosophila melanogaster, the blood-brain barrier is formed by glial cells during late embryogenesis. This protocol describes methods to assay for blood-brain barrier formation and maintenance in D. melanogaster embryos and third instar larvae.