Facilitating Cerebral Organoid Culture via Lateral Soft Light Illumination

2.1K Views

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09:10 min

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June 6th, 2022

DOI :

10.3791/63989-v

June 6th, 2022

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Bangzhu Chen¹^,², Qinrong Lin¹^,², Nengqing Liu¹^,², Diyu Chen¹^,², Yinghui Zhang³, Xiaofang Sun¹^,²

¹Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, ²Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, ³School of Biotechnology and Health Sciences, Wuyi University

Summary

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Cerebral Organoid Culture

Lateral Soft Light Illumination

Cell Detachment Solution

EB Formation Medium

Rho Kinase Inhibitor Y27632

Automatic Culture Machines

Specialized Culture Techniques

Chapters in this video

0:04

Introduction

0:51

Induced Pluripotent Stem Cells (iPSCs) and EB Preparation (Days 0 1)

2:54

Soft Light Lamp Preparation (Day 1), EB Transfer, and Medium Replacement (Days 2 5)

4:24

Neural Induction (Days 5 7)

5:25

Embedding in the Basement Membrane Matrix (Days 7 10)

6:52

Organoid Maturation (Days 10 40)

7:21

Results: Analysis of Cerebral Organoids

8:41

Conclusion

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