Table of Contents

Archive: Issue 61, March 2012

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Bioengineering(7)
Biology(20)
Immunology and Infection(10)
Medicine(7)
Neuroscience(10)
Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry

Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry

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Journal (Bioengineering)

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

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Journal (Bioengineering)

Creating Transient Cell Membrane Pores Using a Standard Inkjet Printer

Creating Transient Cell Membrane Pores Using a Standard Inkjet Printer

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Journal (Bioengineering)

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells

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Journal (Bioengineering)

Alginate Microcapsule as a 3D Platform for Propagation and Differentiation of Human Embryonic Stem Cells (hESC) to Different Lineages

Alginate Microcapsule as a 3D Platform for Propagation and Differentiation of Human Embryonic Stem Cells (hESC) to Different Lineages

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Journal (Bioengineering)

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

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Journal (Bioengineering)

Preparation of 3D Fibrin Scaffolds for Stem Cell Culture Applications

Preparation of 3D Fibrin Scaffolds for Stem Cell Culture Applications

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Journal (Bioengineering)

Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein (GST-RhoA(G17A)) from Epithelial Cell Lysates

Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein (GST-RhoA(G17A)) from Epithelial Cell Lysates

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Journal (Biology)

Fruit Volatile Analysis Using an Electronic Nose

Fruit Volatile Analysis Using an Electronic Nose

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Journal (Biology)

Isolation and Biophysical Study of Fruit Cuticles

Isolation and Biophysical Study of Fruit Cuticles

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Journal (Biology)

Sexual Development and Ascospore Discharge in <em>Fusarium graminearum</em>

Sexual Development and Ascospore Discharge in Fusarium graminearum

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Journal (Biology)

<em>In vivo</em> Electroporation of Morpholinos into the Regenerating Adult Zebrafish Tail Fin

In vivo Electroporation of Morpholinos into the Regenerating Adult Zebrafish Tail Fin

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Journal (Biology)

Endothelial Cell Co-culture Mediates Maturation of Human Embryonic Stem Cell to Pancreatic Insulin Producing Cells in a Directed Differentiation Approach

Endothelial Cell Co-culture Mediates Maturation of Human Embryonic Stem Cell to Pancreatic Insulin Producing Cells in a Directed Differentiation Approach

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Journal (Biology)

Endurance Training Protocol and Longitudinal Performance Assays for <em>Drosophila melanogaster</em>

Endurance Training Protocol and Longitudinal Performance Assays for Drosophila melanogaster

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Journal (Biology)

<em>In vitro</em> Transcription and Capping of <em>Gaussia</em> Luciferase mRNA Followed by HeLa Cell Transfection

In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection

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Journal (Biology)

Chromatin Isolation by RNA Purification (ChIRP)

Chromatin Isolation by RNA Purification (ChIRP)

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Journal (Biology)

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

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Journal (Biology)

Chromatin Immunoprecipitation (ChIP) using <em>Drosophila</em> tissue

Chromatin Immunoprecipitation (ChIP) using Drosophila tissue

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Journal (Biology)

Determining the Contribution of the Energy Systems During Exercise

Determining the Contribution of the Energy Systems During Exercise

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Journal (Biology)

Vampiric Isolation of Extracellular Fluid from <em>Caenorhabditis elegans</em>

Vampiric Isolation of Extracellular Fluid from Caenorhabditis elegans

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Journal (Biology)

Expression Analysis of Mammalian Linker-histone Subtypes

Expression Analysis of Mammalian Linker-histone Subtypes

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Journal (Biology)

The ITS2 Database

The ITS2 Database

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Journal (Biology)

Application of MassSQUIRM for Quantitative Measurements of Lysine Demethylase Activity

Application of MassSQUIRM for Quantitative Measurements of Lysine Demethylase Activity

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Journal (Biology)

Expression and Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein in <em>Saccharomyces cerevisiae</em>

Expression and Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein in Saccharomyces cerevisiae

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Journal (Biology)

Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions

Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions

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Journal (Biology)

Growth Assays to Assess Polyglutamine Toxicity in Yeast

Growth Assays to Assess Polyglutamine Toxicity in Yeast

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Journal (Biology)

March 2012: This Month in JoVE

March 2012: This Month in JoVE

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Journal (Biology)

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

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Journal (Immunology and Infection)

Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology

Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology

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Journal (Immunology and Infection)

Customization of <em>Aspergillus niger</em> Morphology Through Addition of Talc Micro Particles

Customization of Aspergillus niger Morphology Through Addition of Talc Micro Particles

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Journal (Immunology and Infection)

Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens

Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens

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Journal (Immunology and Infection)

High and Low Throughput Screens with Root-knot Nematodes <em>Meloidogyne spp.</em>

High and Low Throughput Screens with Root-knot Nematodes Meloidogyne spp.

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Journal (Immunology and Infection)

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers

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Journal (Immunology and Infection)

Use of Interferon-&gamma; Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

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Journal (Immunology and Infection)

Glass Wool Filters for Concentrating Waterborne Viruses and Agricultural Zoonotic Pathogens

Glass Wool Filters for Concentrating Waterborne Viruses and Agricultural Zoonotic Pathogens

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Journal (Immunology and Infection)

Colonization of <em>Euprymna scolopes</em> Squid by <em>Vibrio fischeri</em>

Colonization of Euprymna scolopes Squid by Vibrio fischeri

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Journal (Immunology and Infection)

Simple and Robust <em>in vivo</em> and <em>in vitro</em> Approach for Studying Virus Assembly

Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly

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Journal (Immunology and Infection)

Eye Tracking Young Children with Autism

Eye Tracking Young Children with Autism

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Journal (Medicine)

Assessing Teratogenic Changes in a Zebrafish Model of Fetal Alcohol Exposure

Assessing Teratogenic Changes in a Zebrafish Model of Fetal Alcohol Exposure

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Journal (Medicine)

Clinical Testing and Spinal Cord Removal in a Mouse Model for Amyotrophic Lateral Sclerosis (ALS)

Clinical Testing and Spinal Cord Removal in a Mouse Model for Amyotrophic Lateral Sclerosis (ALS)

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Journal (Medicine)

MRI-guided Disruption of the Blood-brain Barrier using Transcranial Focused Ultrasound in a Rat Model

MRI-guided Disruption of the Blood-brain Barrier using Transcranial Focused Ultrasound in a Rat Model

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Journal (Medicine)

Chronic Constriction of the Sciatic Nerve and Pain Hypersensitivity Testing in Rats

Chronic Constriction of the Sciatic Nerve and Pain Hypersensitivity Testing in Rats

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Journal (Medicine)

Cholesterol Efflux Assay

Cholesterol Efflux Assay

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Journal (Medicine)

Combination Radiotherapy in an Orthotopic Mouse Brain Tumor Model

Combination Radiotherapy in an Orthotopic Mouse Brain Tumor Model

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Journal (Medicine)

Preparation of Parasagittal Slices for the Investigation of Dorsal-ventral Organization of the Rodent Medial Entorhinal Cortex

Preparation of Parasagittal Slices for the Investigation of Dorsal-ventral Organization of the Rodent Medial Entorhinal Cortex

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Journal (Neuroscience)

Dissection of Adult Mouse Utricle and Adenovirus-mediated Supporting-cell Infection

Dissection of Adult Mouse Utricle and Adenovirus-mediated Supporting-cell Infection

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Journal (Neuroscience)

Dissection and Culture of Mouse Dopaminergic and Striatal Explants in Three-Dimensional Collagen Matrix Assays

Dissection and Culture of Mouse Dopaminergic and Striatal Explants in Three-Dimensional Collagen Matrix Assays

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Journal (Neuroscience)

The Analysis of Purkinje Cell Dendritic Morphology in Organotypic Slice Cultures

The Analysis of Purkinje Cell Dendritic Morphology in Organotypic Slice Cultures

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Journal (Neuroscience)

Inducing Dendritic Growth in Cultured Sympathetic Neurons

Inducing Dendritic Growth in Cultured Sympathetic Neurons

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Journal (Neuroscience)

Transretinal ERG Recordings from Mouse Retina: Rod and Cone Photoresponses

Transretinal ERG Recordings from Mouse Retina: Rod and Cone Photoresponses

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Journal (Neuroscience)

Calcium Imaging of Odor-evoked Responses in the <em>Drosophila</em> Antennal Lobe

Calcium Imaging of Odor-evoked Responses in the Drosophila Antennal Lobe

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Journal (Neuroscience)

Methods to Assay <em>Drosophila</em> Behavior

Methods to Assay Drosophila Behavior

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Journal (Neuroscience)

A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain

A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain

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Journal (Neuroscience)

Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents

Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents

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Journal (Neuroscience)