JoVE Table of Contents: Issue 178, December 2021

<em>In Vitro</em> Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays

In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays

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Rivista (Biochemistry)

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Methods Collections

Purification of Endogenous <em>Drosophila</em> Transient Receptor Potential Channels

Purification of Endogenous Drosophila Transient Receptor Potential Channels

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Rivista (Biochemistry)

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Methods Collections

Modeling an Enzyme Active Site using Molecular Visualization Freeware

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Rivista (Biochemistry)

Monitoring Protein Aggregation Kinetics <em>In Vivo</em> using Automated Inclusion Counting in <em>Caenorhabditis elegans</em>

Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans

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Rivista (Biochemistry)

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Methods Collections

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

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Rivista (Bioengineering)

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Methods Collections

All-optical Mechanobiology Interrogation of Yes-associated Protein in Human Cancer and Normal Cells using a Multi-functional System

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Rivista (Bioengineering)

<em>In vivo</em> Imaging of Biological Tissues with Combined Two-Photon Fluorescence and Stimulated Raman Scattering Microscopy

In vivo Imaging of Biological Tissues with Combined Two-Photon Fluorescence and Stimulated Raman Scattering Microscopy

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Rivista (Bioengineering)

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Methods Collections

Quantitative MRI of Endothelial Permeability and (Dys)function in Atherosclerosis

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Rivista (Bioengineering)

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Methods Collections

Non-thermal Infrared Light Treatment of Ischemia/Reperfusion Injury and Subsequent Analysis of Macrophage Differentiation

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Rivista (Biology)

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Methods Collections

Tobacco Hornworm as an Insect Model System for Cannabinoid Pre-clinical Studies

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Rivista (Biology)

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Methods Collections

Transverse Fracture of the Mouse Femur with Stabilizing Pin

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Rivista (Biology)

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Methods Collections

A Robust Method for the Large-Scale Production of Spheroids for High-Content Screening and Analysis Applications

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Rivista (Biology)

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using <em>In Vivo</em> Inhibition, Immunofluorescence and Flow Cytometry

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

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Rivista (Biology)

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Methods Collections

Rapid <em>In Vivo</em> Fixation and Isolation of Translational Complexes from Eukaryotic Cells

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells

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Rivista (Biology)

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Methods Collections

Determination of Tripartite Interaction between Two Monomers of a MADS-box Transcription Factor and a Calcium Sensor Protein by BiFC-FRET-FLIM Assay

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Rivista (Biology)

Protocol for Analysis and Consolidation of TrackMate Outputs for Measuring Two-Dimensional Cell Motility using Nuclear Tracking

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Rivista (Biology)

Versatility of Protocols for Resistance Training and Assessment Using Static and Dynamic Ladders in Animal Models

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Rivista (Biology)

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Methods Collections

Isolation and Characterization of Mouse Primary Liver Sinusoidal Endothelial Cells

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Rivista (Biology)

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Methods Collections

Circulating Tumor Cell Lines: an Innovative Tool for Fundamental and Translational Research

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Rivista (Cancer Research)

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Methods Collections

An Automated Microscopic Scoring Method for the γ-H2AX Foci Assay in Human Peripheral Blood Lymphocytes

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Rivista (Cancer Research)

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Methods Collections

Using Alizarin Red Staining to Detect Chemically Induced Bone Loss in Zebrafish Larvae

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Rivista (Developmental Biology)

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Methods Collections

Applications of RNA Interference in American Cockroach

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Rivista (Developmental Biology)

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Methods Collections

Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System

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Rivista (Developmental Biology)

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Methods Collections

Bioprospecting of Extremophilic Microorganisms to Address Environmental Pollution

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Rivista (Environment)

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Methods Collections

Low-Cost Automated Flight Intercept Trap for the Temporal Sub-Sampling of Flying Insects Attracted to Artificial Light at Night

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Rivista (Environment)

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Methods Collections

Determining the Role of Maternally-Expressed Genes in Early Development with Maternal Crispants

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Rivista (Genetics)

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Methods Collections

C-section of Preclinical Animal Model of Chorioamnionitis Triggered by Group B <em>Streptococcus</em> (GBS)

C-section of Preclinical Animal Model of Chorioamnionitis Triggered by Group B Streptococcus (GBS)

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Rivista (Immunology and Infection)

Primed Mycobacterial Uveitis (PMU) as a Model for Post-Infectious Uveitis

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Rivista (Immunology and Infection)

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Methods Collections

Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation

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Rivista (Medicine)

An Epithelial Abrasion Model for Studying Corneal Wound Healing

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Rivista (Medicine)

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Methods Collections

Isolation of Adipose Derived Regenerative Cells for the Treatment of Erectile Dysfunction Following Radical Prostatectomy

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Rivista (Medicine)

Culture and Imaging of Human Nasal Epithelial Organoids

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Rivista (Medicine)

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Methods Collections

Locomotor Assessment of 6-Hydroxydopamine-induced Adult Zebrafish-based Parkinson's Disease Model

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Rivista (Neuroscience)

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Methods Collections

Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina

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Rivista (Neuroscience)

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Methods Collections

Expanding the Toolkit for <em>In Vivo</em> Imaging of Axonal Transport

Expanding the Toolkit for In Vivo Imaging of Axonal Transport

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Rivista (Neuroscience)

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Methods Collections

Osmotic Pump-based Drug-delivery for <em>In Vivo</em> Remyelination Research on the Central Nervous System

Osmotic Pump-based Drug-delivery for In Vivo Remyelination Research on the Central Nervous System

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Rivista (Neuroscience)

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Methods Collections

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

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Rivista (Biochemistry)

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Methods Collections

Efficient and Scalable Production of Full-length Human Huntingtin Variants in Mammalian Cells using a Transient Expression System

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Rivista (Biochemistry)

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

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Rivista (Biochemistry)

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Methods Collections

A Novel Tenorrhaphy Suture Technique with Tissue Engineered Collagen Graft to Repair Large Tendon Defects

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Rivista (Bioengineering)

A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation

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Rivista (Bioengineering)

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Methods Collections

Employing Aeroponic Systems for the Clonal Propagation of Cannabis

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Rivista (Bioengineering)

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Methods Collections

Spinal Cord Transection In <em>Xenopus laevis</em> Tadpoles

Spinal Cord Transection In Xenopus laevis Tadpoles

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Rivista (Biology)

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Methods Collections

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting

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Rivista (Biology)

A Step-By-Step Method to Detect Neutralizing Antibodies Against AAV using a Colorimetric Cell-Based Assay

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Rivista (Biology)

Inherent Dynamics Visualizer, an Interactive Application for Evaluating and Visualizing Outputs from a Gene Regulatory Network Inference Pipeline

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Rivista (Biology)

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Methods Collections

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

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Rivista (Biology)

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Methods Collections

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy

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Rivista (Cancer Research)

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Methods Collections

Synthesis of Metal Nanoparticles Supported on Carbon Nanotube with Doped Co and N Atoms and its Catalytic Applications in Hydrogen Production

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Rivista (Chemistry)

Visualizing Cytoskeleton-Dependent Trafficking of Lipid-Containing Organelles in <em>Drosophila</em> Embryos

Visualizing Cytoskeleton-Dependent Trafficking of Lipid-Containing Organelles in Drosophila Embryos

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Rivista (Developmental Biology)

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Methods Collections

Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors

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Rivista (Developmental Biology)

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Methods Collections

Modeling Paracrine Noncanonical Wnt Signaling <em>In Vitro</em>

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

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Rivista (Developmental Biology)

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Methods Collections

Generation of Induced Pluripotent Stem Cells from Turner Syndrome (45XO) Fetal Cells for Downstream Modelling of Neurological Deficits Associated with the Syndrome

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Rivista (Developmental Biology)

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Methods Collections

Rapid Repetition Rate Fluctuation Measurement of Soliton Crystals in a Microresonator

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Rivista (Engineering)

Determination of Total Lipid and Lipid Classes in Marine Samples

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Rivista (Environment)

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Methods Collections

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats

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Rivista (Environment)

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Methods Collections

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

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Rivista (Genetics)

Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4<sup>+</sup> T Cells to Immunodeficient Mice

Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4⁺ T Cells to Immunodeficient Mice

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Rivista (Medicine)

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Methods Collections

Biological Preparation and Mechanical Technique for Determining Viscoelastic Properties of Zonular Fibers

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Rivista (Medicine)

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Methods Collections

Microsurgical Skills of Establishing Permanent Jugular Vein Cannulation in Rats for Serial Blood Sampling of Orally Administered Drug

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Rivista (Medicine)

Aggravation of Myocardial Ischemia upon Particulate Matter Exposure in Atherosclerosis Animal Model

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Rivista (Medicine)

Time-Resolved, Dynamic Computed Tomography Angiography for Characterization of Aortic Endoleaks and Treatment Guidance <em>via</em> 2D-3D Fusion-Imaging

Time-Resolved, Dynamic Computed Tomography Angiography for Characterization of Aortic Endoleaks and Treatment Guidance via 2D-3D Fusion-Imaging

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Rivista (Medicine)

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Methods Collections

An Integrated (Microscopic/Endoscopic) Dissection Ear Surgery Course

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Rivista (Medicine)

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Methods Collections

Application of <em>Lucilia sericata</em> Larvae in Debridement of Pressure Wounds in Outpatient Settings

Application of Lucilia sericata Larvae in Debridement of Pressure Wounds in Outpatient Settings

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Rivista (Medicine)

A Modified Simple Method for Induction of Myocardial Infarction in Mice

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Rivista (Medicine)

Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules

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Rivista (Neuroscience)

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Methods Collections

Endovascular Perforation Model for Subarachnoid Hemorrhage Combined with Magnetic Resonance Imaging (MRI)

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Rivista (Neuroscience)

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Methods Collections

Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy

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Rivista (Neuroscience)

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Methods Collections

Two Peeling Methods for the Isolation of Photoreceptor Cell Compartments in the Mouse Retina for Protein Analysis

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Rivista (Neuroscience)

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Methods Collections

Rapid Golgi Stain for Dendritic Spine Visualization in Hippocampus and Prefrontal Cortex

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Rivista (Neuroscience)

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Methods Collections

Registration of Calcium Transients in Mouse Neuromuscular Junction with High Temporal Resolution using Confocal Microscopy

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Rivista (Neuroscience)

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Methods Collections

Indice

Archivio: Issue 178, December 2021

VAI A :

In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays

Purification of Endogenous Drosophila Transient Receptor Potential Channels

Modeling an Enzyme Active Site using Molecular Visualization Freeware

Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

All-optical Mechanobiology Interrogation of Yes-associated Protein in Human Cancer and Normal Cells using a Multi-functional System

In vivo Imaging of Biological Tissues with Combined Two-Photon Fluorescence and Stimulated Raman Scattering Microscopy

Quantitative MRI of Endothelial Permeability and (Dys)function in Atherosclerosis

Non-thermal Infrared Light Treatment of Ischemia/Reperfusion Injury and Subsequent Analysis of Macrophage Differentiation

Tobacco Hornworm as an Insect Model System for Cannabinoid Pre-clinical Studies

Transverse Fracture of the Mouse Femur with Stabilizing Pin

A Robust Method for the Large-Scale Production of Spheroids for High-Content Screening and Analysis Applications

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells

Determination of Tripartite Interaction between Two Monomers of a MADS-box Transcription Factor and a Calcium Sensor Protein by BiFC-FRET-FLIM Assay

Protocol for Analysis and Consolidation of TrackMate Outputs for Measuring Two-Dimensional Cell Motility using Nuclear Tracking

Versatility of Protocols for Resistance Training and Assessment Using Static and Dynamic Ladders in Animal Models

Isolation and Characterization of Mouse Primary Liver Sinusoidal Endothelial Cells

Culture of Bladder Cancer Organoids as Precision Medicine Tools

Circulating Tumor Cell Lines: an Innovative Tool for Fundamental and Translational Research

An Automated Microscopic Scoring Method for the γ-H2AX Foci Assay in Human Peripheral Blood Lymphocytes

Using Alizarin Red Staining to Detect Chemically Induced Bone Loss in Zebrafish Larvae

Applications of RNA Interference in American Cockroach

Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System

Bioprospecting of Extremophilic Microorganisms to Address Environmental Pollution

Low-Cost Automated Flight Intercept Trap for the Temporal Sub-Sampling of Flying Insects Attracted to Artificial Light at Night

Determining the Role of Maternally-Expressed Genes in Early Development with Maternal Crispants

C-section of Preclinical Animal Model of Chorioamnionitis Triggered by Group B Streptococcus (GBS)

Primed Mycobacterial Uveitis (PMU) as a Model for Post-Infectious Uveitis

Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation

An Epithelial Abrasion Model for Studying Corneal Wound Healing

Isolation of Adipose Derived Regenerative Cells for the Treatment of Erectile Dysfunction Following Radical Prostatectomy

Culture and Imaging of Human Nasal Epithelial Organoids

Locomotor Assessment of 6-Hydroxydopamine-induced Adult Zebrafish-based Parkinson's Disease Model

Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina

Expanding the Toolkit for In Vivo Imaging of Axonal Transport

Osmotic Pump-based Drug-delivery for In Vivo Remyelination Research on the Central Nervous System

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Efficient and Scalable Production of Full-length Human Huntingtin Variants in Mammalian Cells using a Transient Expression System

Determining Total Protein and Bioactive Protein Concentrations in Bovine Colostrum

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

A Novel Tenorrhaphy Suture Technique with Tissue Engineered Collagen Graft to Repair Large Tendon Defects

A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation

Employing Aeroponic Systems for the Clonal Propagation of Cannabis

Spinal Cord Transection In Xenopus laevis Tadpoles

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting

A Step-By-Step Method to Detect Neutralizing Antibodies Against AAV using a Colorimetric Cell-Based Assay

Inherent Dynamics Visualizer, an Interactive Application for Evaluating and Visualizing Outputs from a Gene Regulatory Network Inference Pipeline

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy

Synthesis of Metal Nanoparticles Supported on Carbon Nanotube with Doped Co and N Atoms and its Catalytic Applications in Hydrogen Production

Visualizing Cytoskeleton-Dependent Trafficking of Lipid-Containing Organelles in Drosophila Embryos

Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Generation of Induced Pluripotent Stem Cells from Turner Syndrome (45XO) Fetal Cells for Downstream Modelling of Neurological Deficits Associated with the Syndrome

Rapid Repetition Rate Fluctuation Measurement of Soliton Crystals in a Microresonator

Determination of Total Lipid and Lipid Classes in Marine Samples

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice

Biological Preparation and Mechanical Technique for Determining Viscoelastic Properties of Zonular Fibers

Microsurgical Skills of Establishing Permanent Jugular Vein Cannulation in Rats for Serial Blood Sampling of Orally Administered Drug

Aggravation of Myocardial Ischemia upon Particulate Matter Exposure in Atherosclerosis Animal Model

Time-Resolved, Dynamic Computed Tomography Angiography for Characterization of Aortic Endoleaks and Treatment Guidance via 2D-3D Fusion-Imaging

An Integrated (Microscopic/Endoscopic) Dissection Ear Surgery Course

Application of Lucilia sericata Larvae in Debridement of Pressure Wounds in Outpatient Settings

A Modified Simple Method for Induction of Myocardial Infarction in Mice

Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules

Endovascular Perforation Model for Subarachnoid Hemorrhage Combined with Magnetic Resonance Imaging (MRI)

Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy

Two Peeling Methods for the Isolation of Photoreceptor Cell Compartments in the Mouse Retina for Protein Analysis

Rapid Golgi Stain for Dendritic Spine Visualization in Hippocampus and Prefrontal Cortex

Registration of Calcium Transients in Mouse Neuromuscular Junction with High Temporal Resolution using Confocal Microscopy

Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4⁺ T Cells to Immunodeficient Mice