JoVE Table of Contents: Issue 180, February 2022

A Behavioral Task Modeling 'Everyday Memory' in an Event Arena to Foster Allocentric Representations for Rodents

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Dergi (Behavior)

Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

|

Dergi (Biochemistry)

Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules <em>In Vitro</em> by TIRF Microscopy

Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy

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Dergi (Biochemistry)

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Methods Collections

Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver

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Dergi (Biochemistry)

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

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Dergi (Biochemistry)

|

Methods Collections

CD Spectroscopy to Study DNA-Protein Interactions

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Dergi (Biochemistry)

|

Methods Collections

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

|

Dergi (Biochemistry)

Magnetic Tweezers in a Microplate Format

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Dergi (Biochemistry)

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Methods Collections

The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination

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Dergi (Biochemistry)

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Methods Collections

Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy

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Dergi (Biochemistry)

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Methods Collections

Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy

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Dergi (Biochemistry)

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Methods Collections

Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from <em>S. cerevisiae</em> Cells

Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells

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Dergi (Biochemistry)

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Methods Collections

Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System (MMC)

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Dergi (Bioengineering)

Pattern Generation for Micropattern Traction Microscopy

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Dergi (Bioengineering)

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Methods Collections

Area-based Image Analysis Algorithm for Quantification of Macrophage-fibroblast Cocultures

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Dergi (Bioengineering)

Layer Microdissection of Tricuspid Valve Leaflets for Biaxial Mechanical Characterization and Microstructural Quantification

|

Dergi (Bioengineering)

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Methods Collections

Creation of Patient-Specific Silicone Cardiac Models with Applications in Pre-surgical Plans and Hands-on Training

|

Dergi (Bioengineering)

Voxel Printing Anatomy: Design and Fabrication of Realistic, Presurgical Planning Models through Bitmap Printing

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Dergi (Bioengineering)

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Methods Collections

Construction of Model Lipid Membranes Incorporating G-protein Coupled Receptors (GPCRs)

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Dergi (Bioengineering)

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Methods Collections

Real-Time Intravital Multiphoton Microscopy to Visualize Focused Ultrasound and Microbubble Treatments to Increase Blood-Brain Barrier Permeability

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Dergi (Bioengineering)

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Methods Collections

Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability

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Dergi (Bioengineering)

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Methods Collections

Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis

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Dergi (Bioengineering)

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Methods Collections

Generation of Hook Ischemia-Reperfusion Model using a Three-Day Developing Chick Embryo

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Dergi (Biology)

<em>De novo</em> Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

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Dergi (Biology)

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Methods Collections

A Non-Degradative Extraction Method for Molecular Structure Characterization of Bacterial Glycogen Particles

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Dergi (Biology)

|

Methods Collections

Visualizing Bacterial Motility Based on a Color Reaction

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Dergi (Biology)

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements <em>In Vitro</em>

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

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Dergi (Biology)

Extracellular Vesicle Uptake Assay <em>via</em> Confocal Microscope Imaging Analysis

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

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Dergi (Biology)

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

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Dergi (Biology)

|

Methods Collections

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking

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Dergi (Biology)

|

Methods Collections

Enhancing Tumor Content through Tumor Macrodissection

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Dergi (Biology)

|

Methods Collections

Establishment and Genetic Manipulation of Murine Hepatocyte Organoids

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Dergi (Biology)

|

Methods Collections

Mouse Abdominal Aortic Aneurysm Model Induced by Perivascular Application of Elastase

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Dergi (Biology)

|

Methods Collections

Tension Gauge Tether Probes for Quantifying Growth Factor Mediated Integrin Mechanics and Adhesion

|

Dergi (Biology)

|

Methods Collections

Isolation of Mitochondria from Mouse Skeletal Muscle for Respirometric Assays

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Dergi (Biology)

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Methods Collections

Isolation of Cardiac and Vascular Smooth Muscle Cells from Adult, Juvenile, Larval and Embryonic Zebrafish for Electrophysiological Studies

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Dergi (Biology)

Double-Staining Method to Detect Pectin in Plant-Fungus Interaction

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Dergi (Biology)

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Methods Collections

Isolation and Characterization of the Immune Cells from Micro-dissected Mouse Choroid Plexuses

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Dergi (Biology)

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Methods Collections

Isolation and Characterization of Cyanobacterial Extracellular Vesicles

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Dergi (Biology)

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Methods Collections

An Experimental Approach to Investigating Effects of Artificial Light at Night on Free-Ranging Animals: Implementation, Results, and Directions for Future Research

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Dergi (Biology)

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Methods Collections

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

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Dergi (Biology)

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Methods Collections

Natural Transformation, Protein Expression, and Cryoconservation of the Filamentous Cyanobacterium <em>Phormidium lacuna</em>

Natural Transformation, Protein Expression, and Cryoconservation of the Filamentous Cyanobacterium Phormidium lacuna

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Dergi (Biology)

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Methods Collections

Natural Killer (NK) and CAR-NK Cell Expansion Method using Membrane Bound-IL-21-Modified B Cell Line

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Dergi (Cancer Research)

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Methods Collections

Exploring Mitochondrial Energy Metabolism of Single 3D Microtissue Spheroids Using Extracellular Flux Analysis

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Dergi (Cancer Research)

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Methods Collections

3D Printing and <em>In Situ</em> Surface Modification <em>via</em> Type I Photoinitiated Reversible Addition-Fragmentation Chain Transfer Polymerization

3D Printing and In Situ Surface Modification via Type I Photoinitiated Reversible Addition-Fragmentation Chain Transfer Polymerization

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Dergi (Chemistry)

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

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Dergi (Chemistry)

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Methods Collections

Determination of the Photoisomerization Quantum Yield of a Hydrazone Photoswitch

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Dergi (Chemistry)

Stable Aqueous Suspensions of Manganese Ferrite Clusters with Tunable Nanoscale Dimension and Composition

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Dergi (Chemistry)

Development of Organoids from Mouse Pituitary as <em>In Vitro</em> Model to Explore Pituitary Stem Cell Biology

Development of Organoids from Mouse Pituitary as In Vitro Model to Explore Pituitary Stem Cell Biology

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Dergi (Developmental Biology)

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Methods Collections

Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5

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Dergi (Developmental Biology)

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Methods Collections

Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System

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Dergi (Developmental Biology)

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Methods Collections

Stab Wound Injury Model of the Adult Optic Tectum Using Zebrafish and Medaka for the Comparative Analysis of Regenerative Capacity

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Dergi (Developmental Biology)

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Methods Collections

Robust Differentiation of Human iPSCs into a Pure Population of Adipocytes to Study Adipocyte-Associated Disorders

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Dergi (Developmental Biology)

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Methods Collections

Particle Image Velocimetry Investigation of Hemodynamics <em>via</em> Aortic Phantom

Particle Image Velocimetry Investigation of Hemodynamics via Aortic Phantom

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Dergi (Engineering)

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Methods Collections

<em>In vitro</em> Assessment of Aortic Regurgitation Using Four-Dimensional Flow Magnetic Resonance Imaging

In vitro Assessment of Aortic Regurgitation Using Four-Dimensional Flow Magnetic Resonance Imaging

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Dergi (Engineering)

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Methods Collections

Metal-Assisted Electrochemical Nanoimprinting of Porous and Solid Silicon Wafers

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Dergi (Engineering)

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection

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Dergi (Engineering)

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Methods Collections

Pneumatically Driven Microfluidic Platform for Micro-Particle Concentration

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Dergi (Engineering)

An Application for Pairing with Wearable Devices to Monitor Personal Health Status

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Dergi (Environment)

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Methods Collections

Reusable Single Cell for Iterative Epigenomic Analyses

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Dergi (Genetics)

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Methods Collections

Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay

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Dergi (Genetics)

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Methods Collections

Standardized Colon Ascendens Stent Peritonitis in Rats - a Simple, Feasible Animal Model to Induce Septic Acute Kidney Injury

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Dergi (Immunology and Infection)

Use of Crystal Violet to Improve Visual Cytopathic Effect-based Reading for Viral Titration using TCID50 Assays

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Dergi (Immunology and Infection)

Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots

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Dergi (Immunology and Infection)

Detection of Neutralization-sensitive Epitopes in Antigens Displayed on Virus-Like Particle (VLP)-Based Vaccines Using a Capture Assay

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Dergi (Immunology and Infection)

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Methods Collections

Knee Arthrocentesis in Adults

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Dergi (Medicine)

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Methods Collections

3D Imaging of the Liver Extracellular Matrix in a Mouse Model of Non-Alcoholic Steatohepatitis

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Dergi (Medicine)

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Methods Collections

A Real-World High-Intensity Interval Training Protocol for Cardiorespiratory Fitness Improvement

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Dergi (Medicine)

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Methods Collections

In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter

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Dergi (Medicine)

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Methods Collections

A Surgical Model of Heart Failure with Preserved Ejection Fraction in Tibetan Minipigs

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Dergi (Medicine)

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Methods Collections

Evaluation of Capillary and Other Vessel Contribution to Macular Perfusion Density Measured with Optical Coherence Tomography Angiography

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Dergi (Medicine)

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Methods Collections

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

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Dergi (Medicine)

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

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Dergi (Medicine)

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Methods Collections

The Microscopic Transcanal Approach in Stapes Surgery Revisited

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Dergi (Medicine)

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Methods Collections

Transesophageal Atrial Burst Pacing for Atrial Fibrillation Induction in Rats

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Dergi (Medicine)

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Methods Collections

A Porcine Heterotopic Heart Transplantation Protocol for Delivery of Therapeutics to a Cardiac Allograft

Robot-assisted Total Mesorectal Excision and Lateral Pelvic Lymph Node Dissection for Locally Advanced Middle-low Rectal Cancer

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Dergi (Medicine)

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Methods Collections

Cox-Maze IV Procedure Concomitant with Valvular Surgery In Situs Inversus Dextrocardia: A Single-Center Experience in China

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Dergi (Medicine)

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Methods Collections

Repetitive Blood Sampling from the Subclavian Vein of Conscious Rat

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Dergi (Medicine)

Employing the Forced Oscillation Technique for the Assessment of Respiratory Mechanics in Adults

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Dergi (Medicine)

Long-Term Continuous Measurement of Renal Blood Flow in Conscious Rats

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Dergi (Medicine)

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Methods Collections

Echocardiographic Evaluation of Atrial Communications before Transcatheter Closure

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Dergi (Medicine)

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Methods Collections

A Modified Cuff Technique for Mouse Cervical Heterotopic Heart Transplantation Model

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Dergi (Medicine)

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Methods Collections

A Rat Orthotopic Renal Transplantation Model for Renal Allograft Rejection

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Dergi (Medicine)

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Methods Collections

Laparoscopic Pancreatoduodenectomy for Pancreatic Cancer Using <em>In-Situ</em> No-Touch Isolation Technique

Laparoscopic Pancreatoduodenectomy for Pancreatic Cancer Using In-Situ No-Touch Isolation Technique

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Dergi (Medicine)

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Methods Collections

Accumulated High-intensity Interval Training Protocol: A New Approach to Study Health Markers in Wistar Rats

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Dergi (Medicine)

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Methods Collections

Generation and Characterization of Right Ventricular Myocardial Infarction Induced by Permanent Ligation of the Right Coronary Artery in Mice

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Dergi (Medicine)

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Methods Collections

Rodent Heart and Brain Tissue Preparation for Digital Macro Photography after Ischemia-reperfusion

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Dergi (Medicine)

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Methods Collections

<em>In Vivo</em> Methods to Assess Retinal Ganglion Cell and Optic Nerve Function and Structure in Large Animals

In Vivo Methods to Assess Retinal Ganglion Cell and Optic Nerve Function and Structure in Large Animals

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Dergi (Neuroscience)

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Methods Collections

<em>Ex Vivo</em> Optogenetic Interrogation of Long-Range Synaptic Transmission and Plasticity from Medial Prefrontal Cortex to Lateral Entorhinal Cortex

Ex Vivo Optogenetic Interrogation of Long-Range Synaptic Transmission and Plasticity from Medial Prefrontal Cortex to Lateral Entorhinal Cortex

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Dergi (Neuroscience)

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Methods Collections

Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae

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Dergi (Neuroscience)

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Methods Collections

Mechanical Conflict-Avoidance Assay to Measure Pain Behavior in Mice

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Dergi (Neuroscience)

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Methods Collections

Full-Circle Cauterization of Limbal Vascular Plexus for Surgically Induced Glaucoma in Rodents

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Dergi (Neuroscience)

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Methods Collections

Dissection and Immunohistochemistry of the <em>Drosophila</em> Adult Leg to Detect Changes at the Neuromuscular Junction for an Identified Motor Neuron

Dissection and Immunohistochemistry of the Drosophila Adult Leg to Detect Changes at the Neuromuscular Junction for an Identified Motor Neuron

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Dergi (Neuroscience)

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Methods Collections

Measuring Properties of the Membrane Periodic Skeleton of the Axon Initial Segment using 3D-Structured Illumination Microscopy (3D-SIM)

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Dergi (Neuroscience)

Recording Network Activity in Spinal Nociceptive Circuits Using Microelectrode Arrays

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Dergi (Neuroscience)

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Methods Collections

Intracranial Pressure Monitoring In Nontraumatic Intraventricular Hemorrhage Rodent Model

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Dergi (Neuroscience)

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Methods Collections

A Standardized Pipeline for Examining Human Cerebellar Grey Matter Morphometry using Structural Magnetic Resonance Imaging

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Dergi (Neuroscience)

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Methods Collections

İçindekiler

Arşiv: Issue 180, February 2022

ATLAMAK :

A Behavioral Task Modeling 'Everyday Memory' in an Event Arena to Foster Allocentric Representations for Rodents

Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy

Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

CD Spectroscopy to Study DNA-Protein Interactions

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

Magnetic Tweezers in a Microplate Format

The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination

Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy

Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy

Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells

Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System (MMC)

Pattern Generation for Micropattern Traction Microscopy

Area-based Image Analysis Algorithm for Quantification of Macrophage-fibroblast Cocultures

Layer Microdissection of Tricuspid Valve Leaflets for Biaxial Mechanical Characterization and Microstructural Quantification

Creation of Patient-Specific Silicone Cardiac Models with Applications in Pre-surgical Plans and Hands-on Training

Voxel Printing Anatomy: Design and Fabrication of Realistic, Presurgical Planning Models through Bitmap Printing

Construction of Model Lipid Membranes Incorporating G-protein Coupled Receptors (GPCRs)

Real-Time Intravital Multiphoton Microscopy to Visualize Focused Ultrasound and Microbubble Treatments to Increase Blood-Brain Barrier Permeability

Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability

Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis

Generation of Hook Ischemia-Reperfusion Model using a Three-Day Developing Chick Embryo

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

A Non-Degradative Extraction Method for Molecular Structure Characterization of Bacterial Glycogen Particles

Visualizing Bacterial Motility Based on a Color Reaction

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking

Enhancing Tumor Content through Tumor Macrodissection

Establishment and Genetic Manipulation of Murine Hepatocyte Organoids

Mouse Abdominal Aortic Aneurysm Model Induced by Perivascular Application of Elastase

Tension Gauge Tether Probes for Quantifying Growth Factor Mediated Integrin Mechanics and Adhesion

Isolation of Mitochondria from Mouse Skeletal Muscle for Respirometric Assays

Isolation of Cardiac and Vascular Smooth Muscle Cells from Adult, Juvenile, Larval and Embryonic Zebrafish for Electrophysiological Studies

Double-Staining Method to Detect Pectin in Plant-Fungus Interaction

Isolation and Characterization of the Immune Cells from Micro-dissected Mouse Choroid Plexuses

Isolation and Characterization of Cyanobacterial Extracellular Vesicles

An Experimental Approach to Investigating Effects of Artificial Light at Night on Free-Ranging Animals: Implementation, Results, and Directions for Future Research

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

Natural Transformation, Protein Expression, and Cryoconservation of the Filamentous Cyanobacterium Phormidium lacuna

Dynamic Imaging of Chimeric Antigen Receptor T Cells with [18F]Tetrafluoroborate Positron Emission Tomography/Computed Tomography

Natural Killer (NK) and CAR-NK Cell Expansion Method using Membrane Bound-IL-21-Modified B Cell Line

Exploring Mitochondrial Energy Metabolism of Single 3D Microtissue Spheroids Using Extracellular Flux Analysis

3D Printing and In Situ Surface Modification via Type I Photoinitiated Reversible Addition-Fragmentation Chain Transfer Polymerization

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

Determination of the Photoisomerization Quantum Yield of a Hydrazone Photoswitch

Stable Aqueous Suspensions of Manganese Ferrite Clusters with Tunable Nanoscale Dimension and Composition

Development of Organoids from Mouse Pituitary as In Vitro Model to Explore Pituitary Stem Cell Biology

Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5

Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System

Stab Wound Injury Model of the Adult Optic Tectum Using Zebrafish and Medaka for the Comparative Analysis of Regenerative Capacity

Robust Differentiation of Human iPSCs into a Pure Population of Adipocytes to Study Adipocyte-Associated Disorders

Particle Image Velocimetry Investigation of Hemodynamics via Aortic Phantom

In vitro Assessment of Aortic Regurgitation Using Four-Dimensional Flow Magnetic Resonance Imaging

Metal-Assisted Electrochemical Nanoimprinting of Porous and Solid Silicon Wafers

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection

Pneumatically Driven Microfluidic Platform for Micro-Particle Concentration

An Application for Pairing with Wearable Devices to Monitor Personal Health Status

Reusable Single Cell for Iterative Epigenomic Analyses

Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay

Standardized Colon Ascendens Stent Peritonitis in Rats - a Simple, Feasible Animal Model to Induce Septic Acute Kidney Injury

Use of Crystal Violet to Improve Visual Cytopathic Effect-based Reading for Viral Titration using TCID50 Assays

Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots

Detection of Neutralization-sensitive Epitopes in Antigens Displayed on Virus-Like Particle (VLP)-Based Vaccines Using a Capture Assay

Knee Arthrocentesis in Adults

3D Imaging of the Liver Extracellular Matrix in a Mouse Model of Non-Alcoholic Steatohepatitis

A Real-World High-Intensity Interval Training Protocol for Cardiorespiratory Fitness Improvement

In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter

Laparoscopy-endoscopy Cooperative Surgery for the Treatment of Gastric Gastrointestinal Stromal Tumors

A Surgical Model of Heart Failure with Preserved Ejection Fraction in Tibetan Minipigs

Evaluation of Capillary and Other Vessel Contribution to Macular Perfusion Density Measured with Optical Coherence Tomography Angiography

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

The Microscopic Transcanal Approach in Stapes Surgery Revisited

Transesophageal Atrial Burst Pacing for Atrial Fibrillation Induction in Rats

Dynamic Imaging of Chimeric Antigen Receptor T Cells with [¹⁸F]Tetrafluoroborate Positron Emission Tomography/Computed Tomography