JoVE Table of Contents: Issue 184, June 2022

Nonspecific Proteolysis-Based Glycomics Strategy for Bacterial Glycoproteins

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Dergi (Biochemistry)

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Methods Collections

The Peel-Blot Technique: A Cryo-EM Sample Preparation Method to Separate Single Layers From Multi-Layered or Concentrated Biological Samples

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Dergi (Biochemistry)

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Methods Collections

Self-Assembly of Microtubule Tactoids

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Dergi (Biochemistry)

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Methods Collections

Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution

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Dergi (Biochemistry)

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Methods Collections

Using Lipid Nanoparticles for the Delivery of Chemically Modified mRNA into Mammalian Cells

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Dergi (Biochemistry)

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Methods Collections

A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors

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Dergi (Biochemistry)

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Methods Collections

An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation

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Dergi (Biochemistry)

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Methods Collections

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition <em>In Vitro</em>

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro

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Dergi (Biochemistry)

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Methods Collections

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

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Dergi (Biochemistry)

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level

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Dergi (Bioengineering)

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Methods Collections

Optical Trapping of Plasmonic Nanoparticles for <em>In Situ</em> Surface-Enhanced Raman Spectroscopy Characterizations

Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations

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Dergi (Bioengineering)

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Methods Collections

Direct-Contact Co-culture of Astrocytes and Glioblastoma Cells Patterned using Polyelectrolyte Multilayer Templates

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Dergi (Bioengineering)

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Methods Collections

Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics

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Dergi (Bioengineering)

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Methods Collections

Microfluidic Synthesis of Microgel Building Blocks for Microporous Annealed Particle Scaffold

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Dergi (Bioengineering)

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Methods Collections

Interlinked Macroporous 3D Scaffolds from Microgel Rods

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Dergi (Bioengineering)

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Methods Collections

Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy

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Dergi (Bioengineering)

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Methods Collections

Engineering Antiviral Agents <em>via</em> Surface Plasmon Resonance

Engineering Antiviral Agents via Surface Plasmon Resonance

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Dergi (Bioengineering)

Procurement and Decellularization of Rat Hindlimbs Using an <em>Ex Vivo</em> Perfusion-Based Bioreactor for Vascularized Composite Allotransplantation

Procurement and Decellularization of Rat Hindlimbs Using an Ex Vivo Perfusion-Based Bioreactor for Vascularized Composite Allotransplantation

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Dergi (Bioengineering)

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Methods Collections

Multispectral Optoacoustic Tomography for Functional Imaging in Vascular Research

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Dergi (Bioengineering)

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Methods Collections

Tools for Surface Treatment of Silicon Planar Intracortical Microelectrodes

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Dergi (Bioengineering)

Facilitating Cerebral Organoid Culture <em>via</em> Lateral Soft Light Illumination

Facilitating Cerebral Organoid Culture via Lateral Soft Light Illumination

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Dergi (Bioengineering)

Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates

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Dergi (Bioengineering)

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Methods Collections

A Friction Testing-Bioreactor Device for Study of Synovial Joint Biomechanics, Mechanobiology, and Physical Regulation

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Dergi (Bioengineering)

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Methods Collections

Chemical Triphosphorylation of Oligonucleotides

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Dergi (Bioengineering)

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Methods Collections

Rapid Antibody Glycoengineering in Chinese Hamster Ovary Cells

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Dergi (Bioengineering)

Electroporation-Mediated Delivery of Cas9 Ribonucleoproteins and mRNA into Freshly Isolated Primary Mouse Hepatocytes

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Dergi (Bioengineering)

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Methods Collections

Optimization of Transesophageal Atrial Pacing to Assess Atrial Fibrillation Susceptibility in Mice

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Dergi (Biology)

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Methods Collections

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria

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Dergi (Biology)

Deep Fluorescence Observation in Rice Shoots <em>via</em> Clearing Technology

Deep Fluorescence Observation in Rice Shoots via Clearing Technology

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Dergi (Biology)

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Methods Collections

Intravital Subcellular Microscopy of the Mammary Gland

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Dergi (Biology)

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Methods Collections

A Simple Benchtop Filtration Method to Isolate Small Extracellular Vesicles from Human Mesenchymal Stem Cells

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Dergi (Biology)

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Methods Collections

A Fluorescence-Based Assay of Membrane Potential for High-Throughput Functional Study of Two Endogenous Ion Channels in Two Epithelial Cell Lines

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Dergi (Biology)

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Methods Collections

The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective

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Dergi (Biology)

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Methods Collections

Terminal H-reflex Measurements in Mice

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Dergi (Biology)

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Methods Collections

Isolation and Identification of Vascular Endothelial Cells from Distinct Adipose Depots for Downstream Applications

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Dergi (Biology)

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Methods Collections

Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy

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Dergi (Biology)

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Methods Collections

Performing <em>In Vivo</em> and <em>Ex Vivo</em> Electrical Impedance Myography in Rodents

Performing In Vivo and Ex Vivo Electrical Impedance Myography in Rodents

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Dergi (Biology)

Maintaining Laboratory Cultures of <em>Gryllus bimaculatus</em>, a Versatile Orthopteran Model for Insect Agriculture and Invertebrate Physiology

Maintaining Laboratory Cultures of Gryllus bimaculatus, a Versatile Orthopteran Model for Insect Agriculture and Invertebrate Physiology

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Dergi (Biology)

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Methods Collections

Small-Scale Extraction of <em>Caenorhabditis elegans</em> Genomic DNA

Small-Scale Extraction of Caenorhabditis elegans Genomic DNA

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Dergi (Biology)

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Methods Collections

Anesthesia and Intubation of Preadolescent Mouse Pups for Cardiothoracic Surgery

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Dergi (Biology)

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Methods Collections

Methods for Rearing the Parasitoid <em>Ganaspis brasiliensis</em>, a Promising Biological Control Agent for the Invasive <em>Drosophila suzukii</em>

Methods for Rearing the Parasitoid Ganaspis brasiliensis, a Promising Biological Control Agent for the Invasive Drosophila suzukii

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Dergi (Biology)

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Methods Collections

Three-Dimensional, Serum-Free Culture System for Lacrimal Gland Stem Cells

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Dergi (Biology)

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Methods Collections

Assessment of Open Probability of the Mitochondrial Permeability Transition Pore in the Setting of Coenzyme Q Excess

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Dergi (Biology)

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Methods Collections

ATAC-Seq Optimization for Cancer Epigenetics Research

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Dergi (Cancer Research)

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Methods Collections

Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples

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Dergi (Cancer Research)

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Methods Collections

Robotic Pancreatoduodenectomy for Pancreatic Head Cancer: a Case Report of a Standardized Technique

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Dergi (Cancer Research)

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Methods Collections

<em>In Vivo</em> Imaging to Measure Spontaneous Lung Metastasis of Orthotopically-injected Breast Tumor Cells

In Vivo Imaging to Measure Spontaneous Lung Metastasis of Orthotopically-injected Breast Tumor Cells

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Dergi (Cancer Research)

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Methods Collections

Non-Destructive Evaluation of Regional Cell Density Within Tumor Aggregates Following Drug Treatment

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Dergi (Cancer Research)

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Methods Collections

Pooled shRNA Library Screening to Identify Factors that Modulate a Drug Resistance Phenotype

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Dergi (Cancer Research)

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Methods Collections

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

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Dergi (Cancer Research)

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Methods Collections

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

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Dergi (Cancer Research)

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Methods Collections

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment

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Dergi (Cancer Research)

CAM-Delam Assay to Score Metastatic Properties by Quantifying Delamination and Invasion Capacity of Cancer Cells

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Dergi (Cancer Research)

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Methods Collections

Catalytic Reactions at Amine-Stabilized and Ligand-Free Platinum Nanoparticles Supported on Titania During Hydrogenation of Alkenes and Aldehydes

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Dergi (Chemistry)

Preparation of Enantiopure Non-Activated Aziridines and Synthesis of Biemamide B, D, and <em>epiallo</em>-Isomuscarine

Preparation of Enantiopure Non-Activated Aziridines and Synthesis of Biemamide B, D, and epiallo-Isomuscarine

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Dergi (Chemistry)

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Methods Collections

A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes

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Dergi (Chemistry)

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Methods Collections

Thermochemical Studies of Ni(II) and Zn(II) Ternary Complexes Using Ion Mobility-Mass Spectrometry

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Dergi (Chemistry)

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Methods Collections

Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures

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Dergi (Developmental Biology)

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Methods Collections

Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice

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Dergi (Developmental Biology)

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Methods Collections

Preparation of Single-Somite Explants from Zebrafish Embryos

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Dergi (Developmental Biology)

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Methods Collections

<em>In Ovo</em> and <em>Ex Ovo</em> Methods to Study Avian Inner Ear Development

In Ovo and Ex Ovo Methods to Study Avian Inner Ear Development

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Dergi (Developmental Biology)

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Methods Collections

Fixation of Embryonic Mouse Tissue for Cytoneme Analysis

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Dergi (Developmental Biology)

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Methods Collections

Real Time and Repeated Measurement of Skeletal Muscle Growth in Individual Live Zebrafish Subjected to Altered Electrical Activity

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Dergi (Developmental Biology)

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Methods Collections

Generation of Human Patient iPSC-derived Retinal Organoids to Model Retinitis Pigmentosa

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Dergi (Developmental Biology)

Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells

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Dergi (Developmental Biology)

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Methods Collections

Detection and Quantitation of Label-Retaining Cells in Mouse Incisors using a 3D Reconstruction Approach after Tissue Clearing

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Dergi (Developmental Biology)

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Methods Collections

FACS-Isolation and Culture of Fibro-Adipogenic Progenitors and Muscle Stem Cells from Unperturbed and Injured Mouse Skeletal Muscle

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Dergi (Developmental Biology)

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Methods Collections

Co-localizing Kelvin Probe Force Microscopy with Other Microscopies and Spectroscopies: Selected Applications in Corrosion Characterization of Alloys

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Dergi (Engineering)

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Methods Collections

Sample Preparation and Experimental Design for In Situ Multi-Beam Transmission Electron Microscopy Irradiation Experiments

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Dergi (Engineering)

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

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Dergi (Engineering)

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Methods Collections

Determining Viral Disinfection Efficacy of Hot Water Laundering

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Dergi (Engineering)

Modeling and Experimental Analysis of the Single-Shaft Coaxial Motor-Pump Assembly in Electrohydrostatic Actuators

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Dergi (Engineering)

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Methods Collections

A Modeling and Simulation Method for Preliminary Design of an Electro-Variable Displacement Pump

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Dergi (Engineering)

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Methods Collections

Enhanced Oil Recovery using a Combination of Biosurfactants

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Dergi (Environment)

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Methods Collections

Resource Recycling of Red Soil to Synthesize Fe<sub>2</sub>O<sub>3</sub>/FAU-type Zeolite Composite Material for Heavy Metal Removal

Resource Recycling of Red Soil to Synthesize Fe₂O₃/FAU-type Zeolite Composite Material for Heavy Metal Removal

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Dergi (Environment)

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Methods Collections

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

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Dergi (Genetics)

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Methods Collections

Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling

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Dergi (Genetics)

Isolation and Processing of Murine White Adipocytes for Transcriptome and Epigenome Analyses

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Dergi (Genetics)

Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated CRISPR/Cas9 Gene Editing

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Dergi (Genetics)

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Methods Collections

Reverse Genetics to Engineer Positive-Sense RNA Virus Variants

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Dergi (Genetics)

Measuring <em>Caenorhabditis elegans</em> Sensitivity to the Acetylcholine Receptor Agonist Levamisole

Measuring Caenorhabditis elegans Sensitivity to the Acetylcholine Receptor Agonist Levamisole

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Dergi (Genetics)

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Methods Collections